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Issue 5 (1) 2006 pp. 39-45
Danuta Kulpa, Danuta Rzepka-Plevne, Tomasz Dolat
INITIATION OF IN VITRO CULTURES OF PINUS SYLVESTRIS L.
Abstract The aim of this study was to determine the effects of medium composition and the amount of plant growth regulators essential for shoot initiation in the mature seeds culture of Pinus sylvestris L. For seed germination the following media were used: Whites medium, Murashige and Skoogs medium [MS], a half of MS nutrients, B5 [according to Gamborg et al. 1968], Tisserats medium [cited in Bhojwani and Razdan 1990] and DCR [Gupta i Durzan 1985] marked by the symbols A-N. The above mentioned media combinations differed in the amount of cytokinin BAP (0.25-4.0 mgˇdm-3) and tidiazuron TDZ (0.05-1.0 mgˇdm-3) and auxin IBA (0.5 mgˇdm-3). Pine shoots on DCR medium without growth regulators were the control sample. It was found that for Pine sylvestris, the Tisserats medium [cited in Bhojwani and Razdan 1990] without growth regulators is the most useful medium for shoots initiation during a germination test.
Keywords: Pinus sylvestris, in vitro culture, mature seeds
|MLA||Kulpa, Danuta, et al. "INITIATION OF IN VITRO CULTURES OF PINUS SYLVESTRIS L.." Acta Sci.Pol. Silv. 5.1 (2006): .|
|APA||Danuta Kulpa, Danuta Rzepka-Plevne, Tomasz Dolat (2006). INITIATION OF IN VITRO CULTURES OF PINUS SYLVESTRIS L.. Acta Sci.Pol. Silv. 5 (1),|
|ISO 690||KULPA, Danuta, RZEPKA-PLEVNE, Danuta, DOLAT, Tomasz. INITIATION OF IN VITRO CULTURES OF PINUS SYLVESTRIS L.. Acta Sci.Pol. Silv., 2006, 5.1: .|
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